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1994-10-25
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Document 3040
DOCN M94A3040
TI Viral phenotype and clinical outcome in HIV+ children.
DT 9412
AU Munoz-Fernandez MA; Navarro J; Obregon E; Garcia D; Gurbindo D;
Sampelayo T; Fernandez-Cruz E; Dept. Immunology, H. Gregorio Maranon,
Madrid, Spain.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):167 (abstract no. PB0095). Unique
Identifier : AIDSLINE ICA10/94369535
AB OBJECTIVE: To investigate whether highly replicative HIV-1 isolates in
children has the ability to induce syncytium formation (SI) in PBMC and
MT-2 cells, whereas slow/low viruses lack this capacity. METHODS:
Sixty-four infants born of seropositive HIV mother were studied. Virus
isolation was performed by cocultivation of the patient's PBMC cells
with donor lymphocytes. Viral phenotype: PHA-stimulated PBMC were
infected with cell-free supernatants from the virus cocultures (VC).
After 7-10 days the infected PBMC were cultured with MT-2 cells and HIV
were characterized for SI, and p24 Ag was quantified sequentially in VC
supernatants. RESULTS: Only 14 out of 64 (22%) infants were positive by
PCR and VC. HIV isolates were available in 13 out of 14 infected
children. Of these 13 isolates, 6 were classified as rapid/high and 7 as
slow/low virus. Highly replicative HIV isolates induced syncytia in PBMC
and MT-2 cells, whereas slow/low virus did not. DISCUSSION AND
CONCLUSIONS: Infants with rapid/high isolates will have a more rapid
progressive course during the first months of life and higher levels of
p24 antigenemia, while infants with slowly replicating HIV will do
better.
DE Giant Cells/MICROBIOLOGY Human HIV Core Protein p24/BLOOD HIV
Seropositivity/CLASSIFICATION/*DIAGNOSIS/MICROBIOLOGY
HIV-1/*PATHOGENICITY Infant Infant, Newborn Prognosis Slow Virus
Diseases/CLASSIFICATION/DIAGNOSIS/MICROBIOLOGY Virulence Virus
Replication/*PHYSIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).